ELI-RO 10/2024 Project
English (Engleză)
Project title: Understanding FLASH irradiation effect on cancer cells using laser-induced very high energy electrons and in vitro tumor-on-chip assays / FLASH CANCELLS
Periode: 11 July 2024 – 31 March 2027
Contact Person:
Project Director: Dr. Felix SIMA (INFLPR)
Coordinator:
National Institute for Laser, Plasma and Radiation Physics - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, Romania
Partner:
Institutul de Biochimie al Academiei Romane (IB-AR)
Splaiul Independentei, nr. 296, sector 6, 060031, Bucharest
Abstract (Objectives):
The main objective of this project is to propose and exploit various innovative in vitro approaches in order to understand the FLASH effect induced by either laser-plasma accelerated very high energy electrons (LPA VHEE) or X-ray radiation on cancer cells. To this aim, we will investigate radiation dose dependent cancer cell death vs. potential immune boosting properties, using a panel of chemo- and radio- sensitive or resistant cancer cells together with immune and tissue-specific non-malignant normal cells. Specifically, the project aims to increase the understanding of molecular mechanisms involved in cellular response to FLASH radiation by providing fundamental knowledge of its effects using in vitro models of melanoma and ovarian cancer. A tumour-on-chip model will be proposed for testing the immune response upon co-culture of lymphocytes with cancer spheroids. The tumour-on-chip concept will be designed to test the interaction of cancer cells with artificial narrow tissue-like architectures and immune system components and live monitored by time lapse microscopy in parallel for cancer cells exposed to FLASH irradiation vs. unexposed cancer cells. Other specific objectives refer to i) the development of an X-ray FLASH experimental demonstrator for ultra-high dose rate delivery exceeding 40 Gy/s, ii) the improvement of LPA VHEE experiment reliability by efficient control of laser peak intensity and focus position iii) the development of an LPA geometric configuration to obtain VHEE beams with very high charge and iv) the development of new dosimetric characterization protocols.
Phases:
- 2024 - Analysis of cancer cell response upon exposure to pulsed X-ray and conventional X-ray irradiation conditions;
- 2025 - Analysis of cancer cell response upon exposure to FLASH X-ray irradiation conditions;
- 2026 - Analysis of cancer cell response upon exposure to LPA VHEE for FLASH irradiation and potential synergy with immunotherapeutic (biological) agents;
- 2027 - Validation of proteome analysis identified cancer cell secreted biomarkers of radiotherapeutic response using cell biology and biochemical assays.
Română (Romanian)
Titlul proiectului: Intelegerea efectului de iradiere FLASH asupra celulelor canceroase utilizand fascicule de electroni de energie foarte mare accelerati cu laser si analize in vitro tumora-pe-cip / FLASH CANCELLS
Perioada: 11 Iulie 2024 – 31 Martie 2027
Persoana de contact:
Director Proiect: Dr. Felix SIMA (INFLPR)
Coordonator:
Institutul Național pentru Fizica Laserilor, Plasmei și Radiației - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, ROMÂNIA
Partener:
Institutul de Biochimie al Academiei Romane (IB-AR)
Splaiul Independentei, nr. 296, sector 6, 060031, Bucuresti
Abstract (Obiective):
Obiectivele principale ale acestui proiect sunt propunerea și exploatarea diferitelor abordări in vitro cu scopul de a înțelege efectul FLASH indus asupra celulelor canceroase atât de electronii de energii foarte înalte (very high energy electrons – VHEE) generați prin accelerare laser-plasma (laser-plasma accelerated – LPA), cât și de radiația X. Astfel, propunem investigarea mecanismelor de apoptoză celulară în funcție de doza de iradiere vs. potențiale proprietăți de stimulare imună, folosind celule canceroase rezistente sau sensibile radiologic și chimic, împreună cu celule imune si celule non-maligne sănătoase, specifice tesutului de origine. În mod specific, proiectul propune înțelegerea mecanismelor moleculare implicate în răspunsul celular la radiație FLASH prin furnizarea de cunoștințe fundamentale asupra efectului acesteia folosind modele in vitro de cancer de melanom și cancer ovarian. Un model tumoral pe biocip va fi propus pentru testarea răspunsului imun pe modele de co-cultură de limfocite cu sferoizi tumorali. Conceptul de model tumoral pe biocip va fi elaborat pentru testarea interacției celulelor canceroase cu arhitecturi artificiale constrictive ce mimează țesuturile și cu componente ale sistemului imun si va fi monitorizat prin microscopie în timp real, atât în cazul celulelor expuse la iradieri FLASH, cât și în cazul celulelor neexpuse. Alte obiective specifice se referă la i) dezvoltarea unui demonstrator experimental de raze X de tip FLASH pentru doze ultra-înalte de peste 40 Gy/s, ii) îmbunătățirea fiabilității experimentelor bazate pe VHEE generați prin LPA prin controlul precis al intensității de vârf a laserului și poziția de focus a acestuia, iii) dezvoltarea unei configurații geometrice LPA pentru obținerea fasciculelor VHEE de sarcină foarte înaltă și iv) dezvoltarea de noi protocoale de caracterizare dozimetrică.
Etape:
- 2024 - Analiza răspunsului celulelor canceroase dupa expunerea la diverse condiții de radiație X pulsată și convențională;
- 2025 - Analiza răspunsului celulelor canceroase dupa expunerea la diverse condiții de radiație X de tip FLASH;
- 2026 - Analiza răspunsului celulelor canceroase la expunerea cu electroni VHEE LPA pentru iradieri FLASH și a potențialei sinergii cu agenți imunoterapeutici (biologici);
- 2027 - Validarea analizei proteomice realizate pe biomarkeri secretați de celule canceroase ca răspuns la radioterapie folosind experimente de biologie celulară și biochimie.
RESULTS:
Publications:
[1] S. Orobeti, I. Porosnicu, A. Bran, I. Tiseanu, F. Sima, S. M. Petrescu, and L. E. Sima, "Streamlined Quantification of p-γ-H2AX Foci for DNA Damage Analysis in Melanoma and Melanocyte Co-cultures Exposed to FLASH Irradiation Using Automated Image Cytometry", Bio-protocol 15(4): e5208, 2025.
[2] A. Bran, S. Orobeti, F. Jipa, A. Bonciu, E. Axente, L. E. Sima, F. Sima, K. Sugioka, Tissue-like scaffolds created by two photon polymerization for testing cancer cell behavior in confined environments, accepted for publication, ACS Applied Bio Materials, doi:10.1021/acsabm.5c01009, 2025.
[3] L. E. Sima, M. Tudor, S. Orobeti, A. Filimon, G. Negroiu, C. Munteanu, G. Chiritoiu, A. Roseanu, P. Florian, M. Icriverzi, I. Porosnicu, D. Avram, A. Bran, S. Simion, I.Tiseanu, F. Sima, "Ultra-high dose rate ionizing radiation for FLASH radiotherapy applications", Chapter 6 in the Book "Ionizing Radiation - Insights into Chemistry, Biology, and Applications", Ed. Intech, ISBN978-1-83634-058-4, DOI: 10.5772/intechopen.1010060 (https://www.intechopen.com/chapters/1203856#), 2025.
Patents:
Conference presentations:
[1] L. Ionel, F. Jipa, A. Bran, E. Axente, G. Popescu-Pelin, F. Sima, K. Sugioka, Numerical and experimental investigations of ultra-short pulsed laser interaction with matter in focal region for advanced lab-on-chip applications, 16th International Conference on Physics of Advanced Materials (ICPAM-16), Invited presentation, Akdeniz University in Antalya, Turkey, September 7-15, 2024.
[2] L.E. Sima, S. Orobeti, I. Porosnicu, C. Diplasu, G. Giubega, G. Cojocaru, R. Ungureanu, C. Dobrea, M. Serbanescu, A. Mihalcea, E. Stancu, C. E. Staicu, F. Jipa, A. Bran, E. Axente, S. Sandel, M. Zamfirescu, I. Tiseanu, F. Sima, First molecular investigations using laser-induced high-energy electron FLASH irradiation to characterize cancer cells response to ultrahigh dose rate exposure, The Annual International Conference of the RSBMB 2024, Keynote presentation, Romanian Academy Library, Bucharest, Romania, September 25-27, 2024.
[3] A. Bran, S. Orobeti, L.E. Sima, F. Jipa, A. Bonciu, E. Axente, K. Sugioka, F. Sima, 3D tissue-like scaffolds fabricated by two-photon polymerization for melanoma cell invasion evaluation, The Annual International Conference of the RSBMB 2024, Oral presentation, Romanian Academy Library, Bucharest, Romania, September 25-27, 2024.
[4] S. Orobeti, G. Chirițoiu, M. Tudor, A. Bran, F. Sima, S. Petrescu, L.E. Sima, SKOV3 actin-GFP ovarian cancer stable cell lines for 4D imagistic analysis of cancer cell processes upon treatment with chemo- and FLASH radiotherapy, The Annual International Conference of the RSBMB 2024, Poster presentation, Romanian Academy Library, Bucharest, Romania, September 25-27, 2024.
[5] F. Sima, A. Bran, F. Jipa, L. Ionel, S. Orobeti, E. Axente, L. E. Sima, K. Obata, M. Hanzawa, H. Kawano, K. Sugioka, Tumor-on-chip models for testing cancer cell behavior in tridimensional environments, Congresul Universităţii de Medicină și Farmacie „Carol Davila” din București, XII-a ediție, poster and oral presentation, 24-26 October 2024, Bucuresti, Romania
[6] M. Tudor, C. Munteanu, G. Chiritoiu, S. Orobeti, F. Jipa, F. Sima, G. E. Schiltz, D. Matei, L. Sima, Small molecule inhibitors targeting TG2 in ovarian cancer and identification of drug escape pathways, Congresul Universităţii de Medicină și Farmacie „Carol Davila” din București, XII-a ediție, poster and oral presentation, 24-26 October 2024, Bucuresti, Romania.
[7] L.E. Sima, S. Orobeti#, I. Porosnicu, C. Diplasu, G. Giubega, G. Cojocaru, R. Ungureanu, C. Dobrea, M. Serbanescu, A. Mihalcea, E. Stancu, C.E. Staicu, F. Jipa, A. Bran, E. Axente, S. Sandel, M. Zamfirescu, I. Tiseanu, F. Sima, First in vitro tests of laser-induced very high energy electron FLASH irradiation effect on cancer cells, Invited presentation, OncoHub 2024, November 21-23, 2024, Hotel Pullman, Bucharest, Romania.
[8] S. Orobeti, G. Chiritoiu, M. Tudor, A. Bran, F. Sima, S. Petrescu, L.E. Sima, Developing in vitro models for real-time imaging analysis of cancer cell processes in response to chemotherapeutics and radiation exposure, Poster presentation, 4th edition of OncoHub 2024, November 21-23, 2024, Hotel Pullman, Bucharest, Romania.
[9] F. Sima, A. Bran, K. Obata, M. Hanzawa, H. Kawano, F. Jipa, L. Ionel, S. Orobeti, L. E. Sima, E. Axente, K. Sugioka, Ultrafast laser 3D processing of tumor-on-chip platforms for cancer research, Invited presentation, 5th International Conference on Optics, Photonics and Lasers, 25-27 November 2024, Valencia, Spain.
[10] A. Bran, F. Jipa, A. Bonciu, S. Orobeti, E. Axente, L. E. Sima, F. Sima, K. Sugioka, Cancer cells interaction with 3D tissue-like scaffolds fabricated by two photon polymerization, Oral presentation, 5th International Conference on Optics, Photonics and Lasers, 25-27 November 2024, Valencia, Spain.
[11] F. Sima, K. Obata, M. Hanzawa, H. Kawano, A. Bran, F. Jipa, S. Orobeti, E. Axente, L. E. Sima, K. Sugioka, Ultrafast laser processing of transparent materials for testing cancer cell behavior in tumor-on-chip models, Oral presentation, Photonics West, 24-31, January, 2025, San Francisco, USA.
[12] A. Bran, F. Jipa, A. Bonciu, S. Orobeti, E. Axente, L. E. Sima, F. Sima, K. Sugioka, Engineering complex tissue-like scaffolds by two photon polymerization for testing cancer cell behavior in confined environments, Oral presentation, Photonics West, 24-31, January, 2025, San Francisco, USA.
[13] L.E. Sima, S. Chen, H. Cardenas, G. Zhao, Y. Wang, C. Ivan, H. Huang, B. Zhang, D. Matei, Tissue transglutaminase as a new immunomodulatory target in ovarian cancer tumor microenvironment, Invited presentation, Cancer ImmunoTherapy & ImmunoMonitoring Conference (CITIM) 2025, International Hotel, March 31 – April 3, 2025, Bucharest, Romania.
[14] L.E. Sima, S. Chen, H. Cardenas, G. Zhao, Y. Wang, C. Ivan, H. Huang, B. Zhang, D. Matei, Tissue transglutaminase as a new immunomodulatory target in ovarian cancer tumor microenvironment, Cancer ImmunoTherapy & ImmunoMonitoring Conference (CITIM) 2025, International Hotel, March 31 – April 3, 2025, Bucharest, Romania (invited speaker&poster).
[15] M. Tudor, C. Munteanu, G. Chiritoiu, S. Orobeti, A. Bran, F. Jipa, F. Sima, G.E. Schiltz, D. Matei, LE Sima, Small molecule inhibitors targeting TG2 in ovarian cancer and identification of drug escape pathways, poster presentation, AACR2025, April 25-30, 2025, Chicago, IL, USA.
[16] L.E. Sima, Image and flow cytometry evaluation of cancer cell response to radiation treatment, The 18th National Congress of Cytometry, May 8-9, 2025, Hilton Garden Inn Hotel, Bucharest, Romania (invited speaker).
[17] A. Bran, Tumor-on-chip assays for evaluation of irradiation effects on cancer cells, Oral presentation, Advanced Research Training in Nuclear Photonics / ARNPhot, IRTG-Days, 22 - 23 May 2025, Magurele, Romania.
[18] A. Bran, Confined spaces made by two photon polymerization for cancer cell behavior evaluation, Oral and poster presentation, "Women in Photonics", Career Workshop 1-5, June, 2025, Jena, Germany.
[19] F. Sima, K. Obata, M. Hanzawa, H. Kawano, A. Bran, F. Jipa, L. Ionel, S. Orobeti, L. E. Sima, E. Axente, K. Sugioka, 3D tumour-on-chip models developed by laser processing to test cancer cell behaviour, Oral presentation, The 9th International Congress on Laser Advanced Materials Processing, 10-13, June, 2025, Ise, Japan.
[20] A. Bran, F. Jipa, A. Bonciu, S. Orobeti, E. Axente, L. E. Sima, F. Sima, K. Sugioka, Two photon polymerization of tissue-like scaffolds: a new approach for evaluating cancer cell behaviour in confined 3D environments, Oral presentation, The 9th International Congress on Laser Advanced Materials Processing, 10-13, June, 2025, Ise, Japan.
[21] L.E. Sima, S. Orobeti, I. Dinca, M. Tudor, A. Filimon, G. Negroiu, C. Diplasu, G. Giubega, G. Cojocaru, R. Ungureanu, C. Dobrea, M. Serbanescu, M. Mihalcea, E. Stancu, C.E. Staicu, F. Jipa, A. Bran, E. Axente, S. Sandel, M. Zamfirescu, I. Tiseanu, F. Sima, Laser‑induced high‑energy electron FLASH irradiation of cancer cells for development of new radiotherapeutic strategies, poster presentation, FEBS Congress 2025, July 5-9, 2025, Istanbul, Turkey.
[22] M. Tudor, C. Munteanu, G. Chiritoiu, S. Orobeti, I. Porosnicu, I. Tiseanu, F. Sima, G.E. Schiltz, D. Matei, L.E. Sima, Targeting tissue transglutaminase to enhance ovarian cancer cells response to different therapeutic strategies, poster presentation, FEBS Congress 2025, July 5-9, 2025, Istanbul, Turkey.
[23] L.E. Sima, Exploration of cellular response to different irradiation regimes for application in oncologic radiotherapy, OncoHub 2025 – "Connecting Scientists and Physicians for Next Generation Cancer Management", November 27-29, Hilton Garden Inn Hotel, Bucharest, Romania (invited speaker).
[24] F. Sima, A. Bran, S. Orobeti, L. E. Sima, I. Porosnicu, C. Diplasu, G. Giubega, G. Cojocaru, R. Ungureanu, C. Dobrea, M. Serbanescu, A. Mihalcea, E. Stancu, C. E. Staicu, F. Jipa, E. Axente, S. Simion, M. Zamfirescu, I. Tiseanu, K. Sugioka, Laser induced FLASH very high-energy electron irradiation for cancer research,(Oral presentation), Photonics West, January 17-22, 2026, San Francisco, USA.
[25] F. Sima, A. Bran, S. Orobeti, L. E. Sima, I. Porosnicu, C. Diplasu, G. Giubega, G. Cojocaru, R. Ungureanu, C. Dobrea, M. Serbanescu, A. Mihalcea, E. Stancu, C. E. Staicu, F. Jipa, E. Axente, S. Simion, M. Zamfirescu, I. Tiseanu, K. Sugioka, High-intensity petawatt laser-driven very high energy electrons for FLASH radiotherapy, (Invited presentation), High-Power Laser Ablation (HPLA), March 16-18, 2026, Santa Fe, USA.
[26] A. Bran, D. Avram, F. Jipa, A. Bonciu, C. Dobrea, E. Stancu, S. Orobeti, E. Axente, L. E. Sima, I. Tiseanu, K. Sugioka, F. Sima, Tridimensional polymeric scaffolds fabricated by two-photon polymerization for testing ionizing radiation effects on cancer cells, (Oral presentation), High-Power Laser Ablation (HPLA), March 16-18, 2026, Santa Fe, USA.
[27] L.E. Sima, Integration of multiplex IHC into ovarian cancer research, (Invited presentation) GEN & PATH & Life Science Symposion, IVth edition, March 19, 2026, Artmark, Bucharest, Romania.
[28] L.E. Sima, S. Orobeti, M. Tudor, A. Bran, I. Dinca, D. Avram, E. Stancu, C.E. Staicu, F. Jipa, E. Axente, I. Tiseanu, F. Sima, Using FLASH Irradiation to Characterize Cancer Cells Response to Ultrahigh Dose Rate Exposure, (poster presentation), Global Gynecological Oncology Hub, March 26-28, 2026, Parliament Palace, Bucharest, Romania.
[29] M. Tudor, C. Munteanu, G. Chiritoiu, S. Orobeti, A. Bran, I. Dinca, E. Stancu, D. Avram, A. Filimon, I. Tiseanu, F. Sima, G.E. Schiltz, D. Matei, L.E. Sima, Targeting tissue transglutaminase–fibronectin interaction to enhance ovarian cancer response to novel therapies, (poster presentation), Global Gynecological Oncology Hub, March 26-28, 2026, Parliament Palace, Bucharest, Romania.
[30] L.E. Sima, Flow and image cytometry on a quest to decipher cell response to unconventional irradiation regimes, (Invited presentation), The 19th National Congress of Cytometry, May 13-14, 2026, Hilton Garden Inn Hotel, Bucharest, Romania.
[31] F. Sima, A. Bran, K. Obata, H. Kawano, F. Jipa, L. Ionel, S. Orobeti, L.E. Sima, E. Axente, K. Sugioka, Ultrafast laser microprocessing of transparent materials for cancer research, (Invited presentation), 1st Edition of Advances in Optics and Photonics (AdvOptix), May 25-27, 2026, Matsue, Japan.
[32] L. E. Sima, M. Tudor, A. Filimon, S. Orobeti, A. Bran, I. Dinca, D. Avram, C. Dobrea, E. Stancu, C. E. Staicu, F. Jipa, E. Axente, I. Tiseanu, F. Sima, Molecular characterization of cancer cells response to unconventional irradiation radiotherapy schemes, (Oral presentation), Biodynamics: transdisciplinary challanges, June 3-6, 2026, Bucharest, Romania.
[33] A. Bran, D. Avram, F. Jipa, A. Bonciu, C. Dobrea, E. Stancu, S. Orobeti, E. Axente, L. E. Sima, I. Tiseanu, K. Sugioka, F. Sima, Effect of ionizing radiation on melanoma cells at interaction with tissue-like artificial scaffolds, (Oral presentation), Biodynamics: transdisciplinary challanges, June 3-6, 2026, Bucharest, Romania.
[34] A. Bran, D. Avram, F. Jipa, A. Bonciu, C. Dobrea, E. Stancu, S. Orobeti, E. Axente, L. E. Sima, I. Tiseanu, K. Sugioka, F. Sima, Two Photon Polymerization of 3D Porous Structures for Evaluation of X-Ray Radiation Effect on Cancer Cells, (Oral presentation), 3rd International Conference on Laser, Plasma, and Radiation – Science and Technology (ICLPR-ST), June 29 – July 3, 2026, Poiana Brasov, Romania.
Project meetings and seminaries:
[1] Kick-off meeting FLASH CANCELLS 2025, “Understanding FLASH irradiation effect on cancer cells using laser induced very high energy electrons and in vitro tumor on chip assays” – Results of the 2024 phase of the project, April 16, 2025, CETAL, INFLPR, Magurele, Romania.
[2] Midterm meeting FLASH CANCELLS 2026, “Understanding FLASH irradiation effect on cancer cells using laser induced very high energy electrons and in vitro tumor on chip assays” – Results of the 2025 phase of the project, 13 February 2026, CETAL, INFLPR, Magurele, Romania.
Workshop organization:
[1] Organ-on-Chip Workshop & Hands-on demonstrations on chip handling, fluidic setup, and perfusion principles. Introductory lectures covering organ-on-chip technology and its use in oncology, infection biology, and toxicology - Martyn Steiger, Dynamic42 GmbH, Germany, 10 February 2026, CETAL, Seminar Room, Registered participants (33).
[2] LiCellMo instrument demo workshop (for real-time metabolic measurements of irradiated cancer cells and of a macrophage polarization model), organized in collaboration with Elta’90 and PHC, March 3-4, 2026, IB-AR, Bucharest, Romania.
[3] L.E. Sima - Trainer (theory and hands-on practice) and co-organizer of the Flow cytometry School, 10th edition, May 11-13, 2026, ICUB, Bucharest, Romania.
Webinars:
[1] L.E. Sima, Quantification of p-?-H2AX foci in co-cultured cells exposed to radiation, invited Zoom recorded webinar (with Karly Buffa, TissueGnostics, Austria), released on November 2024, (https://tissuegnostics.com/webinar-livia-sima).
Outreach:
[1] L.E. Sima, participating in AFIST 2024 – topic: „Passion and progress. Women who inspire STEM fields”, November 27, 2024, organized by the Association of Women in Engineering, Science, and Technology (AFIST) under the auspices of CNR UNESCO, Invited interview, House of Trade and Industry, Bucharest (CCIB), Romania;
[2] Livia Sima - Tutor of the workshop “STEM and Biochemistry” at the International Conference of the project “Gender and STEM Education in Romania” organized by the Deutsche Schule Hermann Oberth in partnership with AFIST, moderated by Corina Negrea (Radio Cultural), February 6, 2025;
[3] Livia Sima - Representation of the Institute of Biochemistry of the Romanian Academy, MedFest 2025, University Politechnica of Bucharest, February 26, 2025.
[4] Livia Sima - Tutor of the workshop „STEM and Biochemistry / Biomedical Research” as part of the international conference „Researchers’ Day: Interactive Dialogues, STEM & Artificial Intelligence Workshops – Students and Scientists Collaborating for the Future.”, organized by the Deutsche Schule Hermann Oberth in partnership with AFIST, moderated by Corina Negrea (Radio Cultural), February 6, 2026.
Trainings:
[1] Alexandra Bran, Working stage at Center for Advanced Photonics, RIKEN, 4-27 October, RIKEN, Wako, Japan; Felix Sima, Working visit at Center for Advanced Photonics, RIKEN, 4-14 October, RIKEN, Wako, Japan
[2] Ioana Porosnicu, The XII Geant4 International School, 25-29 November 2024, ELI-NP, Magurele, Romania
[3] Elena Stancu, EURADOS Annual Meeting 2025, EURADOS School on “Metrology in radiation dosimetry measurements” and Training Course “Bonner Sphere Unfolding Refresher Course”, 24-28 February, Bucharest, Romania
[4] Laura Ionel, Winter College on Optics: Theoretical and Applied Aspects of Metamaterials and Metasurfaces, 24 Feb. – 7 Mar. 2025, ICTP, Trieste, Italia;
[5] Alexandra Bran - Career Workshop "Women in Photonics", 1-5, June, 2025, Jena, Germany.
Awards:
[1] Alexandra Bran - Best student oral presentation award “Engineering complex tissue-like scaffolds by two photon polymerization for testing cancer cell behavior in confined environments” – Laser Applications in Microelectronic and Optoelectronic Manufacturing (LAMOM) Ed. XXX, SPIE Photonics West, 24-31, January, 2025, San Francisco, USA.
[2] Alexandra Bran - Travel Grant for the participation at "Women in Photonics", Career Workshop, presentation title “Confined spaces made by two photon polymerization for cancer cell behavior evaluation”, 1-5, June, 2025, Jena, Germany.
[3] Monica Tudor - FEBS Congress Bursary for the participation in FEBS Congress 2025, poster presentation title “Targeting tissue transglutaminase to enhance ovarian cancer cells response to different therapeutic strategies, poster presentation”, July 5-9, 2025, Istanbul, Turkey.
[4] Alexandra Bran - Award of International Society of Electrochemistry for best student oral presentation “Two Photon Polymerization of 3D Porous Structures for Evaluation of X-Ray Radiation Effect on Cancer Cells” – 3rd International Conference on Laser, Plasma, and Radiation – Science and Technology (ICLPR-ST), June 29 – July 3, 2026, Poiana Brasov, Romania.
International Cooperation:
[1] RIKEN, Center for Advanced Laser Photonics, Japan, Professor Koji Sugioka;
[2] Laboratoire Hubert Curien, Université Jean MONNET, France, Dr. Razvan Stoian;