CETAL |PCE Projects (PN-III-P4-IDPCE) | PCE_8/2021

                  ELI-01/2020       
        

    

PCE_8/ 2021 Project


English (Engleză)
Project title: Transparent material processing by nanophotonic technologies for Lab-on-Chip applications / nanoLOC

Periode: 04 Ian 2021 - 29 Dec 2023  

Contact Person:
Project Director: Dr. Felix SIMA (INFLPR)

Coordinator:
National Institute for Laser, Plasma and Radiation Physics - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, Romania
 
Project Summary:
With this project we propose new photonic methods for interaction with various transparent materials using picosecond laser pulses in two processing schemes: subtractive and additive processing. Both fundamental aspects of laser-matter interaction as well as development of technological processes for fabricating miniaturized Lab-on-Chip (LOC) devices will be approached. Direct laser writing, followed by wet chemical etching and annealing treatments will be performed to create 3D microfluidic circuits with nano-characteristics inside glass and polymeric materials. We will further characterize cells grown in 3D complex configurations by fluorescence analyses inside LOCs. Finally, we will test our devices with living cells in a 4D environment with a dual microfluidic-imagining station. We will aim LOC applications for both cancer research to study mechanisms of metastasis and Alzheimer disease by developing a blood brain barrier model, respectively. 

Objectives:
The goal of the project is to produce and test miniaturized 3D lab-on-chip (LOC) microsystems for cancer and blood brain barrier (BBB) applications. The main directions in the project are:
i) laser processing of transparent materials for LOC;
ii) LOC microscopic and spectroscopic analyses;
iii) LOC in vitro testing.

Estimated results:
1. Microdevices with nanochannels and pores down to 500 nm diameter;
2. Laser setup with motorized optics for dynamic laser-induced gradient polymerization in 3D transparent microsystems;
3. Integrated LOC cell analysis setup using a dual microfluidic-imaging platform;
4. BBB-on-chip proof-of-concept;
5. Cancer-on-chip proof-of-concept;
6. Defence of at least one PhD thesis.

Research Team:     
        • Dr. Felix Sima.
        • Dr. Emanuel Axente
        • Dr. Florin Jipa
        • PhD student Stefana Orobeti (Iosub)
        • PhD student Cristina Staicu
        
    
    
    

Română (Romanian)
Titlul proiectului: Tehnologii nanofotonice de procesare a materialelor transparente pentru aplicatii Lab-on-Chip / nanoLOC

Perioada: 04 Ian 2020 - 29 Dec 2023 

Persoana de contact:
Director Proiect: Dr. Felix SIMA (INFLPR)
 
Coordonator:
Institutul Național pentru Fizica Laserilor, Plasmei și Radiației - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, ROMÂNIA
 
Rezumat:
In acest proiect propunem noi metode fotonice de interactie cu diverse materiale transparente folosind pulsuri laser cu durate de ordinul picosecundelor in doua scheme de procesare: substractiva si aditiva. Vor fi abordate atat aspectele fundamentale ale proceselor la interactia laser-materie cat si eventualele dezvoltari tehnologice pentru fabricarea unor dispozitive miniaturale  Lab-on-Chip (LOC). Vor fi efectuate experimente de scriere directa cu laser, corodari chimice umede si tratamente termice pentru a crea circuite microfluidice cu caracteristici nano-dimensionale in sticle si polimeri. Vom efectua apoi analize rapide de fluorescenta 3D a unor celule cultivate in structurile fabricate. In final, vom testa dispozitivele cu culturi celulare vii in mediu 4D cu ajutorul unei statii duale de microfluidica si imagistica. Vom urmari cu prioritate doua aplicatii ale LOC privind studiul mecanismelor de metastazare ale celulelor canceroase si respectiv propunerea unui model de tipul bariera hematoencefalica pentru boala Alzheimer.     

Obiective:
Obiectivul principal al proiectului este realizarea si testarea de microsisteme miniaturale 3D de tipul lab-on-chip (LOC) ca modele de laborator pentru studiul cancerului si, respectiv, al barierei hematoencefalice. Directiile principale ale proiectului sunt:
i) procesarea laser a materialelor transparente pentru realizarea de microsisteme LOC;
ii) analize microscopice si spectroscopice ale microsistemelor LOC;
iii) testarea in vitro a sistemelor LOC.
 
Rezultate estimate:
1. Microdispozitive cu nanocanale si pori de dimensiuni reduse pana la 500 nm;
2. Realizarea unui montaj de procesare laser cu optica motorizata pentru fabricarea de structuri polimerice in LOC prin polimerizare laser in gradient;
3. Realizarea unei platforme integrate de analiza celulara in LOC cu sistem dual de imagistica si microfluidica;
4. Demonstrarea unui concept BBB pe cip;
5. Demonstrarea unui concept cancer pe cip;
6. Finalizarea a minim o teza de doctorat.

Echipa de cercetare:     
    • Dr. Felix Sima
    • Dr. Emanuel Axente
    • Dr. Florin Jipa
    • PhD student Stefana Orobeti (Iosub)
    • PhD student Cristina Staicu
    
    

RESULTS:
    
Publications:
[1] F. Sima, K. Sugioka, “Ultrafast laser manufacturing of nanofluidic systems”, Nanophotonics, doi.org/10.1515/nanoph-2021-0159, June, 2021.
[2] C.E. Staicu, F. Jipa, E. Axente, M. Radu, B.M. Radu, F. Sima, “Lab-on-a-Chip Platforms as Tools for Drug Screening in Neuropathologies Associated with Blood–Brain Barrier Alterations”, Biomolecules, 11 (6), 916, 2021.
    

Conference presentations:
[1] F. Sima, H. Kawano, A. Miyawaki, K. Obata, D. Serien, K.Sugioka, Prostate cancer cells retain their cell division ability upon migration through sub-micrometric intravasation-extravasation-like confining spaces, Oral presentation, RIKEN Workshop on Multi-Dimensional Cell Measurement, Online, 24 February 2021.
[2] F. Sima, K. Sugioka, 3D glass nanofluidics fabricated by femtosecond laser processing for study on cancer cell metastasis, On demand Paper 11637-26, Photonics West, Online, 6-11 March, 2021.
[3] F. Sima, S. Orobeti, C. Butnaru, F. Jipa, E. Axente, H. Kawano, A. Miyawaki, K. Obata, D. Serien, K. Sugioka, Ultrafast Laser Processing of Glass Microfluidic Systems: Application to Cancer Research, Invited presentation, XXVIth International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society, virtual mode (Cluj-Napoca, Romania), 9-13 May, 2021.
[4] F. Sima, F. Jipa, C. Butnaru, S. Orobeti, E. Axente, H. Kawano, A. Miyawaki, K. Obata, D. Serien, K. Sugioka, “Hybrid laser technologies for biomimetic material processing”, Invited presentation at 2nd International Summit on Optics, Photonics and Laser Technologies (Optics-2021) (virtual), June 28-30, 2021.