We have recently obtained in vitro results related to 3D biomimetic, electromagnetic active scaffolds for bone regeneration and we want to validate them in vivo, in terms of osseointegration and osteogenic potential. Our motivation relies on the fact that, although our preliminary in vitro tests were encouraging, a realistic estimation of scaffolds ability to promote the bone growth requires a translational in vivo testing model. In this context, EMABON project will develop functional 3D biomimetic, electromagnetic active scaffolds with strong osseointegration and osteogenic potential. The scaffolds will be validated in vivo, by implantation at the site of femoral defects artificially created in skeletally mature rats. The osteogenesis will be promoted via the synergic action of scaffolds biomimetic architecture and remotely applied electromagentic stimuli. Through the proposed methodology and expected results, EMABON reponds to a major issue of public health related to poor bone quality and bone-loss associated conditions such as osteoporosis, diabetes mellitus or chronic kidney disease. The proposed model will also find usefulness for orthopedic surgery goals, such as fracture repair, arthroplasty or plate and screw fixation.

The goal of EMABON project is to validate in vivo an existing experimental demonstrative model that we recently tested in vitro. The experimental demonstrator is represented by 3D biomimetic, electromagnetic active scaffolds for bone regeneration. The end result will consist in scaffolds with better osseointegration and superior osteogenic potential than the existing bone grafts. The project will:
a) Use the knowhow of the team members for developing functional 3D biomimetic, electromagnetic active scaffolds with superior capabilities than the existing bone grafts;
b) Validate our 3D biomimetic, electromagnetic active scaffolds in vivo, in terms of osseintegration and osteogenic potential.
O1. To develop an in vivo model for assessment of functional 3D biomimetic, electromagnetic active scaffolds for bone regeneration.
O2. To validate the scaffolds in vivo, on animal models.
O3. To surpass the performances of existing scaffolds for bone regeneration.

Etapa I a proiectului are ca obiectiv proiectarea procedurilor experimentale in vivo.
Activitatea 1.1. Proiectarea modelului animal
Activitatea 1.2. Proiectarea arhitecturii scafoldurilor active din punct de vedere electromagnetic
Activitatea 1.3. Proiectarea configuratiei electromagnetice
Activitatea 1.4. Fabricarea scafoldurilor active din punct de vedere electromagnetic
Activitatea 1.5. Diseminare-Raport de faza
Etapa a II-a a proiectului are ca obiectiv proiectarea, fabricarea și testarea scaffold-urilor superparamagnetice cu arhitecturi reproductibile pentru stimularea in camp magnetic static (Static Magnetic Field, SMF) a osteogenezei.
Activitatea 2.1. Validarea in vivo a scaffold-urilor active din punct de vedere electromagnetic
Activitatea 2.2. Compararea performantelor scaffold-urilor in vitro si in vivo
Activitatea 2.3. Evaluarea in vivo a performantelor scaffold-urilor

• Dr. Luculescu Catalin
• Dr. Paun Irina
• Dr. Sporea Dan
• Dr. Popescu Andrei
• Drd. Calin Bogdan
• Dr. Porosnicu Corneliu
• Dr. Stochioiu Andrei
• Dr. Acasandrei Adriana
• Dr. Mustaciousu Cosmin
• Dr. Popescu Roxana Cristina
• Dr. Temelie Mihaela
• Dr. Savu Iulia
• Dr. Polifron Victoria

We have recently obtained in vitro results related to 3D biomimetic, electromagnetic active scaffolds for bone regeneration and we want to validate them in vivo, in terms of osseointegration and osteogenic potential. Our motivation relies on the fact that, although our preliminary in vitro tests were encouraging, a realistic estimation of scaffolds ability to promote the bone growth requires a translational in vivo testing model. In this context, EMABON project will develop functional 3D biomimetic, electromagnetic active scaffolds with strong osseointegration and osteogenic potential. The scaffolds will be validated in vivo, by implantation at the site of femoral defects artificially created in skeletally mature rats. The osteogenesis will be promoted via the synergic action of scaffolds biomimetic architecture and remotely applied electromagentic stimuli. Through the proposed methodology and expected results, EMABON reponds to a major issue of public health related to poor bone quality and bone-loss associated conditions such as osteoporosis, diabetes mellitus or chronic kidney disease. The proposed model will also find usefulness for orthopedic surgery goals, such as fracture repair, arthroplasty or plate and screw fixation.

The goal of EMABON project is to validate in vivo an existing experimental demonstrative model that we recently tested in vitro. The experimental demonstrator is represented by 3D biomimetic, electromagnetic active scaffolds for bone regeneration. The end result will consist in scaffolds with better osseointegration and superior osteogenic potential than the existing bone grafts. The project will:
a) Use the knowhow of the team members for developing functional 3D biomimetic, electromagnetic active scaffolds with superior capabilities than the existing bone grafts;
b) Validate our 3D biomimetic, electromagnetic active scaffolds in vivo, in terms of osseintegration and osteogenic potential.
O1. To develop an in vivo model for assessment of functional 3D biomimetic, electromagnetic active scaffolds for bone regeneration.
O2. To validate the scaffolds in vivo, on animal models.
O3. To surpass the performances of existing scaffolds for bone regeneration.

Etapa I a proiectului are ca obiectiv proiectarea procedurilor experimentale in vivo.
Activitatea 1.1. Proiectarea modelului animal
Activitatea 1.2. Proiectarea arhitecturii scafoldurilor active din punct de vedere electromagnetic
Activitatea 1.3. Proiectarea configuratiei electromagnetice
Activitatea 1.4. Fabricarea scafoldurilor active din punct de vedere electromagnetic
Activitatea 1.5. Diseminare-Raport de faza
Etapa a II-a a proiectului are ca obiectiv proiectarea, fabricarea și testarea scaffold-urilor superparamagnetice cu arhitecturi reproductibile pentru stimularea in camp magnetic static (Static Magnetic Field, SMF) a osteogenezei.
Activitatea 2.1. Validarea in vivo a scaffold-urilor active din punct de vedere electromagnetic
Activitatea 2.2. Compararea performantelor scaffold-urilor in vitro si in vivo
Activitatea 2.3. Evaluarea in vivo a performantelor scaffold-urilor

• Dr. Luculescu Catalin
• Dr. Paun Irina
• Dr. Sporea Dan
• Dr. Popescu Andrei
• Drd. Calin Bogdan
• Dr. Porosnicu Corneliu
• Dr. Stochioiu Andrei
• Dr. Acasandrei Adriana
• Dr. Mustaciousu Cosmin
• Dr. Popescu Roxana
• Dr. Temelie Mihaela
• Dr. Savu Iulia
• Dr. Polifron Victoria