PED_596 Project
English (Engleză)
Project title: Optoelectric microfluidic system for tumor cells characterization and separation according to their malignancy grade/ TUMOSIGN
Periode: 21 Jun 2022 - 20 Jun 2024
Partners:
National Institute for Laser, Plasma and Radiation Physics - INFLPR (Coordinator)
University of Medicine and Pharmacy "Carol Davila" Bucharest – UMF (Partner 1)
National Institute of Materials Physics – INCDFM (Partner 2)
Contact Person:
Project Director: Dr. Felix SIMA
Coordinator:
National Institute for Laser, Plasma and Radiation Physics - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, Romania
Project Summary:
In the present project we shall fabricate and validate a microfluidic device based on dielectrophoresis and optical tweezers capable to characterize and separate cancer cells according to their malignancy potency. Specifically, using a novel fabrication approach, we will develop a compact glass based optoelectric biochip for efficient cell trapping followed by microfluidic separation. The device will provide a complete label-free method of cell separation and consists in creating a double trap due to gradients of electric field amplitude and laser light intensity. The sorted cells can be further used for downstream applications (such as, precision medicine diagnostic by providing specific cell subsets or obtaining specific cell cultures for selection of chemotherapy drugs). The device has the perspective to be integrated into an automated theranostic technology or system to evaluate specific cancer cells types and to sort them in designated groups.
Objectives:
The feasibility of the project is ensured by reaching the following Specific Objectives: O1. Opto-electric characterization of cancer cells with different capacity of malignancy. O2. Identification of homogeneous cancer cell populations with similar OEF. O3. Selection and sorting of cancer cells according to their OEF. O4. Validation of cancer cells separation concept and selection procedure based on OEF.
Research Team:
• Prof. Univ. Dr. Tudor Savopol – responsible P1 - UMF
• Dr. George Stan– responsible P2 - INCDFM
• Dr. Emanuel Axente - INFLPR
• Dr. Florin Jipa - INFLPR
• Dr. Cristina Nita - INFLPR
• PhD student Elena-Cristina Staicu - INFLPR
• Dr. Mihaela-Georgeta Moisescu - UMF
• Dr. Ioan-Cristian Tivig - UMF
• Dr. Andreas Rinne - UMF
• PhD student Christien Oktaviani Matei - UMF
• Dr. Cristina Besleaga - INCDFM
• Tech. Catalin Radulescu - INCDFM
Română (Romanian)
Titlul proiectului: Sistem microfluidic opto-electric pentru caracterizarea si separarea celulelor tumorale in functie de gradul de malignitate/ TUMOSIGN
Perioada: 21 Iun 2022 - 20 Iun 2024
Parteneri:
Institutul Național pentru Fizica Laserilor, Plasmei și Radiației – INFLPR (Coordonator)
Universitatea de Medicina si Farmacie "Carol Davila" – UMF (Partener 1)
Institutul National de Cercetare-Dezvoltare pentru Fizica Materialelor - INCDFM (Partener 2)
Persoana de contact:
Director Proiect: Dr. Felix SIMA (INFLPR)
Coordonator:
Institutul Național pentru Fizica Laserilor, Plasmei și Radiației - I.N.F.L.P.R.
Atomiștilor 409, Măgurele, ROMÂNIA
Rezumat:
In acest proiect, propunem fabricarea si validarea unui dispozitiv microfluidic bazat pe dielectroforeza si penseta laser, capabil sa caracterizeze si sa sorteze celule canceroase in functie de potentialul malign. In particular, printr-o noua abordare de fabricare, vom dezvolta un biocip optoelectric pe baza de sticla pentru trapare celulara urmata de separare prin metode microfluidice. Dispozitivul va putea separa populatii celulare printr-o metoda lipsita complet de marcare chimica, prin crearea unei duble capcane celulare rezultate in urma unui gradient al amplitudinii campului electric si respectiv al intensitatii fasciculului laser. Celulele sortate pot fi apoi utilizate pentru aplicatii diverse (precum, obtinerea unui diagnostic precis prin separarea unor subpopulatii celulare specifice sau obtinerea unor culturi celulare specifice pentru selectia citostaticelor in tratamentul cancerului). Dispozitivul are perspective de a fi integrat intr-o tehnologie sau sistem automatizat de medicina teranostica pentru evaluarea unor tipuri de celule canceroase de interes medical si separarea acestora.
Obiective:
Realizarea proiectului este bazata pe urmatoarele obiective specifice: O1. Caracterizarea opto-electrica a celulelor canceroase in functie de potentialul malign. O2. Identificarea populatiilor celulare omogene cu amprenta optoelectrical similara. O3. Selectia si sortarea celulelor canceroase dupa amprenta optoelectrical. O4. Validarea conceptului de separare a celulelor canceroase si a procedurii de selectie pe baza amprentei opto-electrice.
• Prof. Univ. Dr. Tudor Savopol - responsabil partener 1 - UMF
• Dr. George Stan – responsabil partener 2 - INCDFM
• Dr. Emanuel Axente - INFLPR
• Dr. Florin Jipa - INFLPR
• Dr. Cristina Nita - INFLPR
• PhD student Elena-Cristina Staicu - INFLPR
• Dr. Mihaela-Georgeta Moisescu - UMF
• Dr. Ioan-Cristian Tivig - UMF
• Dr. Andreas Rinne - UMF
• PhD student Christien Oktaviani Matei - UMF
• Dr. Cristina Besleaga - INCDFM
• Tech. Catalin Radulescu - INCDFM
Proceedings:
[1] F. Sima, M. Hanzawa, K. Obata, H. Kawano, K. Sugioka, “Processing 3D transparent materials with sub-micron precision by ultra-fast laser technologies: application to cancer research”, Proceedings of LPM2023 – the 24th International Symposium on Laser Precision Microfabrication, 1-4, 2023.
Conference presentations:
[1] F. Jipa, Stefana Orobeti, Cristina Staicu, Emanuel Axente, Hiroyuki Kawano, Koji Sugioka, Felix Sima, “Processing transparent materials with sub-micron precision by ultrafast laser technologies: application to cancer research”, CurvoBio2022 Workshop, Berlin, Germany, (poster presentation), 24-26 August 2022
[2] T. Savopol, I. Tivig, M. G. Moisescu, Order changes of lipids in membranes exposed to electropermeabilization pulses, oral presentation, 17-a Conferință Națională de Biofizică, CNB2022, Tg. Mureș, 23-26 Septembrie 2022
[3] D. Singh-Yadav, I. Tivig, M. G. Mmoisecu, T. Ssavopol, Single-cell opto-electric manipulation for characterization and separation of retinal pigment epithelial cells, poster presentation, 17-a Conferință Națională de Biofizică, CNB2022, Tg. Mureș, 23-26 Septembrie 2022
[4] Ioan Tivig, Tudor Savopol, Mihalea G. Moisescu 3D printing capability in producing specialized devices for biophysics research, poster presentation, 17-a Conferință Națională de Biofizică, CNB2022, Tg. Mureș, 23-26 Septembrie 2022
[5] I. Tivig, M. G. Moisescu, T. Savopol, Electropermeabilization and the packing of bilayer lipids: a problem addressed by real-time fluorescence measurements, oral presentation, 4th World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies Copenhagen, Denmark 9−13 October, 2022
[6] I. Tivig, M. G. Moisescu, E. Kovacs, T. Savopol, Characterization of an experimental setup for recording fluorescence in real-time from a cell membrane exposed to electric pulses, oral presentation, 4th World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies Copenhagen, Denmark 9−13 October, 2022
[7] I. Tivig, “Innovative Lab-on-a-chip systems for the electrical characterization of cells”, Oral presentation, CETAL Workshop (https://lpr-st.ro/evenimente/), May 3, 2023.
[8] F. Sima, F. Jipa, C. Staicu, S. Orobeti, L. Ionel, A. Bran, E. Axente, K. Sugioka, “Transparent material processing by laser technologies for Lab-on-Chip applications”, International Conference on processing & manufacturing of advanced materials: processing, fabrication, properties, applications (Thermec23), Vienna, Austria (invited presentation), July 2 - 7, 2023.
RESEARCH OUTREACH:
Project Open Days: "Cancer-on-chip" experiment prepared and presented live to students and general public during visiting the research institution and lab (Big Brother la microscop: celule canceroase vii observate în nano-acvarii, Saptamana Portilor Deschise la INFLPR, 26-28 septembrie 2022, http://portideschise.inflpr.ro/index.php/experimente/)